Archive for the 'science!' Category

signs

on a billboard on my way to work:

“Do you believe in love at first zip?”

-Earthlink

doesn’t that seem a little dirty for an ISP advertisement?

On a door I pass on my way to the pop machines:

“Please do not open this door. Doing so will compromise our experiments.”

Really? What are you doing in there? It’s not dark, I can see light coming through the foil and cardboard taped on to the window. I am now OBSESSED! What would really happen? What could opening your door possibly do? Are there fearful mutants in there? Why couldn’t you have just put a “keep door closed” sign on the door, and I would have never given it a second thought. But now it’s all I can do to walk past your door WITHOUT EXERTING MASSIVE WILLPOWER TO KEEP MYSELF FROM OPENING YOUR DOOR!!!

Two doors down from previous door:

“Beware of very friendly dog”

What????? Is there a dog in your lab? I’d open the door to check, but I’m afraid that I would compromise your experiments.

On a totally different note, I just screwed up and ruined the experiment I had planned for today. I have something else I can do today, but I felt it best to give myself a little “time out” before I go ballistic and do something crazy. Like open a door.

as published in the Journal of Barf

I have not posted anything of substance lately, I know.

Substance? HA! Like dirty monkey sex stories? pft. you’ve NEVER had anything with substance here.

Shut up.

Anyway…it’s because, as many of you know, I am in the final throes of graduate school. This is the most painful phase, called “writing.” After collecting and incubating years and years worth of data, I now must give birth to an elegantly written explanation, summary, and defense of all that data.

Folks, I think this is a breach birth. or this sucker is coming out sideways. It’s pretty painful.

Actually, I’m not solely working on my thesis, I am working on a paper of my main project in the lab that will be submitted to a journal for publication . the nice thing is, with a few edits and additions of some “eh” data, this paper will also be the bulk of my thesis. Copy. Paste. Print.

The thing that is making this so difficult is that word that can either bring tears to the eyes, or send shivers down the spine of any graduate student: collaboration

A collaboration means that you take your data and someone else’s data (often from another lab, at another school), both of which might be slightly weak on their own, but together make for a stronger paper. You both get 1st author on the paper, and everyone is happy.

Except I’M the one writing this paper. The girl I collaborated with wrote her thesis and is GONE. I have her thesis to work from, and now I must mesh her data and mine into a beautiful tapestry that tells a story and has an easy to follow ending.

Oh, and while our projects are similar (how else could we combine them?), our…research focus is different. Without giving too much away, and to totally confuse those of you who can’t remember 9th grade biology, she worked with a meiosis assay, while mine is mitotic.

Not such a big deal, especially when you’re just writing up the methods, and then describing the data. Everything can be kept separately. here is my stuff, here is her stuff.

BUT…

the discussion section. OH MY GOD, THE DISCUSSION! Where everything has to be wrapped up neatly in a little bundle! Where I find the intersection of my data and hers, and come up with some universal model that explains them both! Genius! Neat! Elegant!

I

.

.

.

CAN’T

.

.

.

DO IT!!!

I’m trying. I really am. But I feel like every time I try to type something scholarly and succinct, what I’m really doing is just barfing all over the screen. Yep, here you go, Dr. Adviser…barf paper! Mind the chunks, they’re nasty!

So that, dear friends, is the reason for my crap posts that are half pictures, and my general absence in commentsville. I do still read you, I promise. But only a few at a time, stolen pieces of time while I eat lunch, or to calm me down and keep me from hurling my laptop at the wall (i’ve come close, believe me).

I remember a talk I had with Brenda, a previous graduate student in the lab and occasional reader here (HI BRENDA!), after she had finished writing her thesis. She said, and I’m paraphrasing here, “You know when Jenny was writing hers and having all those problems, I remember thinking ‘I’m a good writer, this is not going to be so difficult for me.’ But ESC, it IS hard. So very hard. It’s the hardest thing and most painful thing I’ve ever had to write. you might think it will be easy but IT WON’T. IT WILL BE VERY DIFFICULT.”

Brenda, I miss our little pep talks. You always knew how to cheer me up.

So yeah, it’s hard. Vomit inducing hard. And OH MY GOD thank JESUS for pills, because without my happy pills, none of this would be working out for me AT ALL. I just turned in draft #5 (a day late. oops. did I mention how hard the discussion section is?), which is PROGRESS people. PROGRESS!

Now I’m going to go over the draft again and fix all the little typos I made and missed during the 20 reads of the paper before I turned it in, which she will find and mark and send back to me. Because I always do stupid shit like that. I also need more references. Just how many references does one sentence need? 2? 8? I MUST REFERENCE EVERYONE IN HISTORY WHO MENTIONS THIS GENE IN ANY CONTEXT!

No.

Alright, I need food - I think my blood sugar MIGHT be a touch low…ya think?

web karma

I need to stop posting about my freak show of a high school. Let’s review:

Super fertile Kurt

Jesus-finding Jeff

She’s a Survivor Becky

Google karma was sure to catch up with me. So a big HELLO to Jeff the Jerk! Who I suppose is probably not a jerk anymore, despite what I wrote. Let’s all hope none of us are still frozen in our old high school mentality. Good god…I would be…*shudder*…yeah, let’s not go there. Anyway…SORRY I CALLED YOU A JERK! I’m sure Jesus made the right decision to appear in your shower.

Tell your mom I said “hi.” And…you know…uhhh…don’t tell my family about my blog.
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sweet science-fu

So there is this one experiment that has been the bane of my existance for a while now. Why bane? Because 2/3 of the data from my 2nd project HINGE on the results. If the results showed one thing, it would mean reconstructing many strains and redoing all the experiments. If they turned out the OTHER way, it means…DONE! The problem has been just getting the damned thing to work!!!

Many weeks spent trying to get it to work. With my adivsor breathing down my neck, which is NO MEAN FEAT, as she is in NC. “YOU NEED TO GET THIS DONE, ESC! WE CAN’T INTERPRET YOUR DATA WITHOUT IT! YOU KNOW YOU NEED TO DO THIS BEFORE YOU CAN GRADUATE! AND HAVE I MENTIONED THAT YOUR FUNDING WILL BE CUT OFF IN MAY???”

gah!

So yeah…anyway…results today:

you see this?

IT WORKED (trust me)! and NOT ONLY did it work, IT’S THE RIGHT RESULT! I’M DONE BITCH!!!

My advisor’s response via email?

“great! now blah blah other stuff…”

great? GREAT??? IT’S FAN-FUCKING-TASTIC, BITCH!!! My advisor has trouble expressing emotion. Emotion other than disapproval, that is.
*sigh* and there was nobody around to take me out for a drink. Doesn’t matter - I left early, got my hair cut, bought some champagne*, some lipstick**, and I am HOME. Not going back until next week, and ONLY to help pack up the lab and send it off. Then nothing to do but WRITE MY ASS OFF!

after the beach, of course. ahhhh…can’t wait for the beach…
* realized that the girls and I will be getting our hair done around the time we should be eating lunch before the wedding. helpful salon ladies made suggestions. So it’s mimosas and nibbles*** to nosh on while we’re being pampered. It’s a rough life.
**I bought lipstick last week - the “stay on all day” kind - for the wedding. I really liked the color…until I got it home and tried it, and realized that the reason I liked the color so much was that it was the EXACT same color as my lips. so that was a waste of $8.

***hey Cinn, care to donate some caviar? ;)

you look so calm!

Nothing stresses me out more than people telling me how not stressed I look. “are you nervous? You look so calm! I’d be so freaked out right now! HAHAHAHA!!”

Hahahahahafuckyou.

I’m on very good drugs, OK? And for once I think I have things ALMOST under control. Except that the ditz limo guy still hasn’t faxed my mom the payment form, and I still have to pay the cake guy - I’m probably going to have to drop it off in person…SWELL…and there is much confusion at the church office about wedding protocol, as my pastor told me they would use the deposit that they weren’t supposed to cash that they DID cash anyway towards the fees for janitorial services, the organist, etc, but apparently no one told the office manager this and OH MY GOD I hope they get that straightened out, otherwise we are going to owe a lot more money…even though we’d get some of it back..

AND ARE YOU CONFUSED YET, BECAUSE I SURE AM!!!

**************

depressing stereotypes

I sat behind a girl who happened to be african-american on the shuttle today, and noticed her studying a notebook of her handwritten asian writing of some kind. I asked her what language she was studying.

“Japanese”

“wow. I’m really impressed!”

“hehehe thanks! It’s really hard.”

“I’m sure it is!”

I had so much respect for her, trying to learn such a difficult language. I suck at languages - I can’t imagine learning one that’s not even romantic/germanic based.

1 minute later I lost my respect for her as she answered her cell phone.

“hey! where you at?”

GAAAAAAAAAAAAAAAAAAAAAAAH!!!

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geek nostalgia

The contents of the lab are officially moving off to Duke next week, and the lone post-doc and I are scrambling to get things ready. We need to hoard away the stuff we’re keeping, and get everything else cleaned up and ready to move that’s going.

I’m going through the shelves above my bench, pulling out half-empty bottles of media, old no-longer-sterile water, and no-longer-used buffers…when I find…

Buffers I and III. Old school miniprep buffers.

They were also the very first buffers I made in the lab. Probably on the first day - the date on the bottles is right around then. We don’t use them anymore - we have the miracle of pre-made kits. But still…it was a little hard to dump those down the drain.

*******************

*twitch*

For some tradition which origins are unknown to me, and probably are non-existant, Kev and I are…”refraining” for this week leading up to the wedding. Some people do a month, some do two weeks…we think we might make it a week. This is some kind of stupid idea thought up by people like us who have been living IN SIN and think that by abstaining for a few days before the wedding, our virginities will magically reappear and our wedding night can be super duper special.

Which is not only bullshit, but also some kind of cosmic joke, because just when you’re at your MOST stressed and need some kind of outlet…NO SEX FOR YOU!

We kind of cheated last night, but not really. We just…uhhh…made ourselves happy in the vicinity of each other. Bwaha! how’s THAT FOR WORDING!

Anyway…this is going to be a rough week all around.

oy.

ohfridaythankgod

Wednesday I gathered my strength, make some last minute tweaks, took a deep breath, and sent the 2nd draft of my paper off to my advisor.

Then I exhaled. I figured I now had a few days to get some WORK DONE! Thursday I got results from two experiments (one interesting, one failed…AGAIN). I decided that I would spend friday mostly at home, setting up my references in Endnote, reading some relavent papers that come out recently, and most importantly get some WEDDING THINGS DONE! But first get a new battery for my cell phone, because it is dead as a doornail and I am a lost puppy without my cellphone.

Then I got an email at the end of the day thursday. From the advisor. She had read the draft and made her comments IN ONE DAY, was fedexing it to me so I would get it friday morning, take a few hours to look things over, and then call her on the phone at 3 to discuss what changes need to be made.

Fuck.

So there went my friday. I am still without cell phone. And without a limo for the wedding. Both of which I will remedy Satuday, I hope.

At least the conversation with advisor went relatively well. She feels the paper is coming together well and that it should be ready to go very soon. Of course, there are plenty of adjustments to make, collaborator data to include, REFERENCES TO ADD.

I had PLANNED ON DOING THAT TODAY!!!

grrrr.

Mexican food for dinner, though. Mmmmm…